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anti cd69 pe vio770 conjugated  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti cd69 pe vio770 conjugated
    Anti Cd69 Pe Vio770 Conjugated, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd69+pe/CD69+Antibody%2C+anti-mouse/bio_rxiv__64898__2025__12__29__696857-181-70-73
    Average 93 stars, based on 186 article reviews
    anti cd69 pe vio770 conjugated - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Altered X-chromosome inactivation predisposes to autoimmunity
    Article Snippet: Levels of inflammatory cytokines in sera were measured using a cytometric bead array mouse inflammation kit (552364, BD Biosciences) according to the manufacturer’s instructions. .. BM, spleen, blood, and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-allophycocyanin (APC) (130-123-207, Miltenyi Biotec), CD5-APC-Vio770 (130-120-165, Miltenyi Biotec), CD8-fluorescein isothiocyanate (FITC) (130-118-468, Miltenyi Biotec), CD11b APC (553312, BD Pharmingen), CD11c phycoerythrin (PE)–Vio770 (130-110-840, Miltenyi Biotec), CD19-FITC (557398, BD Pharmingen), CD21-APC-Vio770 (130-111-733, Miltenyi Biotec), CD23-PE-Vio770 (130-118-764, Miltenyi Biotec), CD38-PE (130-123-571, Miltenyi Biotec), CD43-PE (130-112-887, Miltenyi Biotec), CD69-PE (130-115-575, Miltenyi Biotec), CD138 PE-Vio615 (130-108-989, Miltenyi Biotec), F4/80 FITC (130-117-509, Miltenyi Biotec), Ter119 PE (130-112-909, Miltenyi Biotec), SiglecH APC-Vio770 (130-112-299, Miltenyi Biotec), B220-APC (130-110-847, Miltenyi Biotec), B220 VioBlue (130-110-851, Miltenyi Biotec), IgM-VioBlue (130-116-318, Miltenyi Biotec), IgD-PE (130-111-496, Miltenyi Biotec), GL7-PE-Cy7 (144619, BioLegend), Ly6C-FITC (130111-915, Miltenyi Biotec), streptavidin FITC (554060, BD Biosciences), CD138 BV605 (563147, BD-Horizon), CD23 BV605 (101637, BioLegend), I-A/I-E BV711 (107643, BioLegend), CD19 BV786 (563333, BD Horizon), T and B cell activation antigen (GL7) PE (561530, BD Pharmingen), CD95 PE-Cy7 (557653, BD Pharmingen), IgM APC-eFluor 780 (47-5790-82, Invitrogen), CD45R/B220 APC/cyanine7 (103224, BioLegend), CD11b eFluor 450 (48-0112-82, eBioscience), CD267 (TACI) BV421 (742840, BD Biosciences), IgD BUV395 (564274, BD Horizon), streptavidin APC (4317-82, eBioscience), CD3 Biotin (100304, BioLegend), Biotin CD11c (568970, BD Biosciences), CD21 PercP Cy5.5 (562797, BD Biosciences), and Fixable Viability Dye eFluor 506 (65-0866-18, Invitrogen) following the recommendations of the manufacturers. .. Flow cytometry was conducted on a BD FACSAria Fusion (BD Biosciences) at the ImagoSeine platform of the Jacques Monod Institute (Paris, France).

    Article Title: Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancer
    Article Snippet: Cells were permeabilized for staining with Ki67 PE-Vio770 (Miltenyi, Catalog# 130-120-419). .. To characterize memory T cell populations CD8 T cells the dispersed cells were surface stained in master mixes of following conjugated antibodies: CD69-PE (Miltenyi, Catalog# 130-115-575), CD3-FITC (Miltenyi, Catalog# 130-119-798), CD8β-PerCPVio700 (Miltenyi, Catalog# 130-111-715), P2X7R-APC (Miltenyi, Catalog# 130-114-330), CD62L-VioBlue (Miltenyi, Catalog# 130-112-841) and CD44-PE-Vio770 (Miltenyi, Catalog# 130-110-085). ..

    Article Title: Assessment of immunostimulatory responses to the antimiR-22 oligonucleotide compound RES-010 in human peripheral blood mononuclear cells
    Article Snippet: .. To this end, 10 5 PBMCs were stained with CD45-PE-Vio770 (Miltenyi Biotec, Cat. N. 130-110-634)and CD3-APC (Miltenyi Biotec, Cat. N. 130-113-135)to select the total population of T-cells and with CD25 APC-Vio770 (Miltenyi Biotec, Cat. N. 130-123-469), CD69-PE (Miltenyi Biotec, Cat. N. 130-112-613) and HLA-DR PerCP-Vio700 (Miltenyi Biotec, Cat. N. 130-111-793) for cell activation assessment. .. T-cell activation was assessed using BD FACSVerse cytometer.

    Article Title: Altered X-chromosome inactivation predisposes to autoimmune manifestations in mice
    Article Snippet: .. Bone marrow, spleen, blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207, Miltenyi Biotec), CD5-APC-Vio770 (130-120-165, Miltenyi Biotec), CD8-FITC (130-118-468, Miltenyi Biotec), CD11b APC (553312, BD Pharmingen), CD11c PE-Vio770 (130-110-840, Miltenyi Biotec), CD19-FITC (557398, BD Pharmingen), CD21-APC-Vio770 (130-111-733, Miltenyi Biotec), CD23-PE-Vio770 (130-118-764, Miltenyi Biotec), CD38-PE (130-123-571, Miltenyi Biotec), CD43-PE (130-112-887, Miltenyi Biotec), CD69-PE (130-115-575, Miltenyi Biotec), CD138 PE-Vio615 (130-108-989, Miltenyi Biotec), F4/80 FITC (130-117-509, Miltenyi Biotec), Ter119 PE (130-112-909, Miltenyi Biotec), SiglecH APC-Vio770 (130-112-299, Miltenyi Biotec), B220-APC (130-110-847, Miltenyi Biotec), B220 VioBlue (130-110-851, Miltenyi Biotec), IgM-VioBlue (130-116-318, Miltenyi Biotec), IgD-PE (130-111-496, Miltenyi Biotec), GL7-PE-Cy7 (144619, BioLegend), Ly6C-FITC (130-111-915, Miltenyi Biotec) following recommendations of the manufacturers. .. Flow cytometry was conducted on a BD FACSAria Fusion (BD Biosciences) at the Imagoseine platform of the Jacques Monod Institute (Paris, France).

    Activation Assay:

    Article Title: Altered X-chromosome inactivation predisposes to autoimmunity
    Article Snippet: Levels of inflammatory cytokines in sera were measured using a cytometric bead array mouse inflammation kit (552364, BD Biosciences) according to the manufacturer’s instructions. .. BM, spleen, blood, and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-allophycocyanin (APC) (130-123-207, Miltenyi Biotec), CD5-APC-Vio770 (130-120-165, Miltenyi Biotec), CD8-fluorescein isothiocyanate (FITC) (130-118-468, Miltenyi Biotec), CD11b APC (553312, BD Pharmingen), CD11c phycoerythrin (PE)–Vio770 (130-110-840, Miltenyi Biotec), CD19-FITC (557398, BD Pharmingen), CD21-APC-Vio770 (130-111-733, Miltenyi Biotec), CD23-PE-Vio770 (130-118-764, Miltenyi Biotec), CD38-PE (130-123-571, Miltenyi Biotec), CD43-PE (130-112-887, Miltenyi Biotec), CD69-PE (130-115-575, Miltenyi Biotec), CD138 PE-Vio615 (130-108-989, Miltenyi Biotec), F4/80 FITC (130-117-509, Miltenyi Biotec), Ter119 PE (130-112-909, Miltenyi Biotec), SiglecH APC-Vio770 (130-112-299, Miltenyi Biotec), B220-APC (130-110-847, Miltenyi Biotec), B220 VioBlue (130-110-851, Miltenyi Biotec), IgM-VioBlue (130-116-318, Miltenyi Biotec), IgD-PE (130-111-496, Miltenyi Biotec), GL7-PE-Cy7 (144619, BioLegend), Ly6C-FITC (130111-915, Miltenyi Biotec), streptavidin FITC (554060, BD Biosciences), CD138 BV605 (563147, BD-Horizon), CD23 BV605 (101637, BioLegend), I-A/I-E BV711 (107643, BioLegend), CD19 BV786 (563333, BD Horizon), T and B cell activation antigen (GL7) PE (561530, BD Pharmingen), CD95 PE-Cy7 (557653, BD Pharmingen), IgM APC-eFluor 780 (47-5790-82, Invitrogen), CD45R/B220 APC/cyanine7 (103224, BioLegend), CD11b eFluor 450 (48-0112-82, eBioscience), CD267 (TACI) BV421 (742840, BD Biosciences), IgD BUV395 (564274, BD Horizon), streptavidin APC (4317-82, eBioscience), CD3 Biotin (100304, BioLegend), Biotin CD11c (568970, BD Biosciences), CD21 PercP Cy5.5 (562797, BD Biosciences), and Fixable Viability Dye eFluor 506 (65-0866-18, Invitrogen) following the recommendations of the manufacturers. .. Flow cytometry was conducted on a BD FACSAria Fusion (BD Biosciences) at the ImagoSeine platform of the Jacques Monod Institute (Paris, France).

    Article Title: Altered X-chromosome inactivation predisposes to autoimmunity.
    Article Snippet: .. 10, eadn6537 (2024) 3 May 2024 11 of 14 (PE)–Vio770 (130- 110- 840, Miltenyi Biotec), CD19- FITC (557398, BD Pharmingen), CD21- APC- Vio770 (130- 111- 733, Miltenyi Biotec), CD23- PE- Vio770 (130- 118- 764, Miltenyi Biotec), CD38- PE (130- 123- 571, Miltenyi Biotec), CD43- PE (130- 112- 887, Miltenyi Biotec), CD69- PE (130- 115- 575, Miltenyi Biotec), CD138 PEVio615 (130- 108- 989, Miltenyi Biotec), F4/80 FITC (130- 117- 509, Miltenyi Biotec), Ter119 PE (130- 112- 909, Miltenyi Biotec), SiglecH APC- Vio770 (130- 112- 299, Miltenyi Biotec), B220- APC (130- 110- 847, Miltenyi Biotec), B220 VioBlue (130- 110- 851, Miltenyi Biotec), IgM- VioBlue (130- 116- 318, Miltenyi Biotec), IgD- PE (130- 111- 496, Miltenyi Biotec), GL7- PE- Cy7 (144619, BioLegend), Ly6C- FITC (130111- 915, Miltenyi Biotec), streptavidin FITC (554060, BD Biosciences), CD138 BV605 (563147, BD- Horizon), CD23 BV605 (101637, BioLegend), I- A/I- E BV711 (107643, BioLegend), CD19 BV786 (563333, BD Horizon), T and B cell activation antigen (GL7) PE (561530, BD Pharmingen), CD95 PE- Cy7 (557653, BD Pharmingen), IgM APC- eFluor 780 (47- 5790- 82, Invitrogen), CD45R/ B220 APC/cyanine7 (103224, BioLegend), CD11b eFluor 450 (48- 0112- 82, eBioscience), CD267 (TACI) BV421 (742840, BD Biosciences), IgD BUV395 (564274, BD Horizon), streptavidin APC (4317- 82, eBioscience), CD3 Biotin (100304, BioLegend), Biotin CD11c (568970, BD Biosciences), CD21 PercP Cy5.5 (562797, BD Biosciences), and Fixable Viability Dye eFluor 506 (65- 0866- 18, Invitrogen) following the recommendations of the manufacturers. .. Flow cytometry was conducted on a BD FACSAria Fusion (BD Biosciences) at the ImagoSeine platform of the Jacques Monod Institute (Paris, France).

    Article Title: Assessment of immunostimulatory responses to the antimiR-22 oligonucleotide compound RES-010 in human peripheral blood mononuclear cells
    Article Snippet: .. To this end, 10 5 PBMCs were stained with CD45-PE-Vio770 (Miltenyi Biotec, Cat. N. 130-110-634)and CD3-APC (Miltenyi Biotec, Cat. N. 130-113-135)to select the total population of T-cells and with CD25 APC-Vio770 (Miltenyi Biotec, Cat. N. 130-123-469), CD69-PE (Miltenyi Biotec, Cat. N. 130-112-613) and HLA-DR PerCP-Vio700 (Miltenyi Biotec, Cat. N. 130-111-793) for cell activation assessment. .. T-cell activation was assessed using BD FACSVerse cytometer.

    Article Title: Donor selection for adoptive cell therapy with CD45RA − memory T cells for patients with coronavirus disease 2019, and dexamethasone and interleukin-15 effects on the phenotype, proliferation and interferon gamma release
    Article Snippet: .. We employed other antibodies for specific cell populations: CD25 BV421 (BD Horizon, Franklin Lakes, NJ, USA) and CD127 PE-CF594 (BD Horizon) for regulatory T cells (Treg); HLA-DR BV421 (BD Pharmingen, San Diego, CA, USA), CD69 PE (Miltenyi Biotec) and CD25 BV421 (BD Horizon) for activation makers; CD279 (PD1) AF700 (BioLegend, San Diego, CA, USA) and NKG2A BV421 (BD OptiBuild) for exhaustion markers; and, CD103 BV421 (BD Horizon) and CCR7 PE-CF594 (BD Horizon) for chemokine receptor and integrin markers. ..

    Article Title: Donor selection for adoptive cell therapy with CD45RA - memory T cells for patients with coronavirus disease 2019, and dexamethasone and interleukin-15 effects on the phenotype, proliferation and interferon gamma release.
    Article Snippet: .. We employed other antibodies for specific cell populations: CD25 BV421 (BD Horizon, Franklin Lakes, NJ, USA) and CD127 PECF594 (BD Horizon) for regulatory T cells (Treg); HLA-DR BV421 (BD Pharmingen, San Diego, CA, USA), CD69 PE (Miltenyi Biotec) and CD25 BV421 (BD Horizon) for activation makers; CD279 (PD1) AF700 (BioLegend, San Diego, CA, USA) and NKG2A BV421 (BD OptiBuild) for exhaustion markers; and, CD103 BV421 (BD Horizon) and CCR7 PE-CF594 (BD Horizon) for chemokine receptor and integrin markers. ..

    other:

    Article Title: Donor selection for adoptive cell therapy with CD45RA − memory T cells for patients with coronavirus disease 2019, and dexamethasone and interleukin-15 effects on the phenotype, proliferation and interferon gamma release
    Article Snippet: In addition to CFSE, cells were stained with the following cell surface antibodies to define different subpopulations: CD27 APC (BD Pharmingen), CD3 Viogreen (Miltenyi Biotec), CD4 PE-Cy7 (BD Pharmingen), CD8 APC-Cy7 (BD Pharmingen), L/D 7AAD (BD Pharmingen), CD45RA Alexa Fluor 700 (BD Pharmingen), CD127 PE-CF594 (BD Horizon) and CD25 BV421 (BD Horizon).



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    (A). (i) Expression of Pf GBP-130 ECD fused with Transferrin membrane domain on the membrane of CHO K1 cells by infecting the lentiviral vector; pMSCV Puro and its immunofluorescence analysis using anti-rabbit Pf GBP antibody. (ii) CHO K1 cells expressing Pf GBP-130 ECD bind LFA-1 αI-Fc. Binding of purified LFA-1 αI-Fc to Pf GBP-130 ECD expressing CHO cells was assessed by FACS using an PE-Texas red anti-human IgG antibody. (B) NK cells activation in the presence of CHO K1 cells expressing Pf GBP ECD. Human NK cells were purified (>95%) from fresh PBMC and co-cultured with CHO K1 cells expressing Pf GBP ECD in 2: 1 ratio (20,000 CHO-K1 cells:10,000 NK cells) and these cells were stimulated with (Poly I:C/lipofectamine 2000) for 24 h. NK cells were separated from adherent CHO K1 cells and NK cells activation was assessed by assaying the expression of activation markers <t>(CD69,</t> CD25) and a degranulation marker; CD107a. NK cells co-cultured with CHO K1 cells expressing Pf GBP-ECD protein showed significant increase in the expression of CD25 and CD69, as well as CD107a in comparison to the NK cells co-cultured with mock CHO cells. Addition of anti-CD11a (HI111 clone) antibodies reduced the expression of both activation and degranulation markers. * Denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001.
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    (A). (i) Expression of Pf GBP-130 ECD fused with Transferrin membrane domain on the membrane of CHO K1 cells by infecting the lentiviral vector; pMSCV Puro and its immunofluorescence analysis using anti-rabbit Pf GBP antibody. (ii) CHO K1 cells expressing Pf GBP-130 ECD bind LFA-1 αI-Fc. Binding of purified LFA-1 αI-Fc to Pf GBP-130 ECD expressing CHO cells was assessed by FACS using an PE-Texas red anti-human IgG antibody. (B) NK cells activation in the presence of CHO K1 cells expressing Pf GBP ECD. Human NK cells were purified (>95%) from fresh PBMC and co-cultured with CHO K1 cells expressing Pf GBP ECD in 2: 1 ratio (20,000 CHO-K1 cells:10,000 NK cells) and these cells were stimulated with (Poly I:C/lipofectamine 2000) for 24 h. NK cells were separated from adherent CHO K1 cells and NK cells activation was assessed by assaying the expression of activation markers (CD69, CD25) and a degranulation marker; CD107a. NK cells co-cultured with CHO K1 cells expressing Pf GBP-ECD protein showed significant increase in the expression of CD25 and CD69, as well as CD107a in comparison to the NK cells co-cultured with mock CHO cells. Addition of anti-CD11a (HI111 clone) antibodies reduced the expression of both activation and degranulation markers. * Denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001.

    Journal: bioRxiv

    Article Title: LFA-1 Interaction with GBP-130 on Plasmodium falciparum -infected Red Blood Cells mediates NK Cell Activation and Parasite Control

    doi: 10.1101/2025.08.20.671208

    Figure Lengend Snippet: (A). (i) Expression of Pf GBP-130 ECD fused with Transferrin membrane domain on the membrane of CHO K1 cells by infecting the lentiviral vector; pMSCV Puro and its immunofluorescence analysis using anti-rabbit Pf GBP antibody. (ii) CHO K1 cells expressing Pf GBP-130 ECD bind LFA-1 αI-Fc. Binding of purified LFA-1 αI-Fc to Pf GBP-130 ECD expressing CHO cells was assessed by FACS using an PE-Texas red anti-human IgG antibody. (B) NK cells activation in the presence of CHO K1 cells expressing Pf GBP ECD. Human NK cells were purified (>95%) from fresh PBMC and co-cultured with CHO K1 cells expressing Pf GBP ECD in 2: 1 ratio (20,000 CHO-K1 cells:10,000 NK cells) and these cells were stimulated with (Poly I:C/lipofectamine 2000) for 24 h. NK cells were separated from adherent CHO K1 cells and NK cells activation was assessed by assaying the expression of activation markers (CD69, CD25) and a degranulation marker; CD107a. NK cells co-cultured with CHO K1 cells expressing Pf GBP-ECD protein showed significant increase in the expression of CD25 and CD69, as well as CD107a in comparison to the NK cells co-cultured with mock CHO cells. Addition of anti-CD11a (HI111 clone) antibodies reduced the expression of both activation and degranulation markers. * Denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001.

    Article Snippet: The following antibodies were used for staining: anti-human CD3 (biotin, OKT3; Elabsciences), anti CD16 (3G8; Biolegend), anti CD11a (HI111; BioLegend), CD56-PE (5.1H11; Elabsciences), Ultra-LEAFTM Purified Human IgG1 Isotype (Biolegend), Goat anti-Human IgG Alexa FluorTM 488 (Invitrogen), Goat anti-Human IgG Secondary Antibody, Alexa FluorTM 594 (Invitrogen), (CD107a (H4A3; Elabsciences), CD25-Alexa Fluor® 488 (BioLegend), and CD69 (FN50; Elabsciences).

    Techniques: Expressing, Membrane, Plasmid Preparation, Immunofluorescence, Binding Assay, Purification, Activation Assay, Cell Culture, Marker, Comparison

    (A) Activated human NK cells eliminate iRBCs in vitro. Human NK cells when co-cultured with iRBCs reduce parasitemia significantly after 96h and in the presence of anti- Pf GBP-130 antibodies this reduction in parasitemia was blocked. Presence of anti-GBP-130 abs resulted in parasitemia simialr to the control when NK cells were incubated with iRBCs alone. (B) NK cells activation in the presence of iRBCs. Human NK cells were were purified (>95%) from fresh PBMC and co-cultured with synchronized schizont stage iRBCs at a parasitemia of 0.5% in 10:1 ratio (NK: iRBC) for 48h. Quantification of activation and degranulation markers was performed after 48 hours. NK cells co-cultured with iRBCs showed significant increase in the expression of CD25 and CD69, the two activation markers as well as for the expression of CD107a, a degranulation marker in comparison to the NK cells co-cultured with RBCs alone. Addition of anti-rabbit PfGBP-130 antibodies reduced the expression of both activation and degranulation markers in these NK cells in comparison to rabbit IgG isotype control. * Denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001.

    Journal: bioRxiv

    Article Title: LFA-1 Interaction with GBP-130 on Plasmodium falciparum -infected Red Blood Cells mediates NK Cell Activation and Parasite Control

    doi: 10.1101/2025.08.20.671208

    Figure Lengend Snippet: (A) Activated human NK cells eliminate iRBCs in vitro. Human NK cells when co-cultured with iRBCs reduce parasitemia significantly after 96h and in the presence of anti- Pf GBP-130 antibodies this reduction in parasitemia was blocked. Presence of anti-GBP-130 abs resulted in parasitemia simialr to the control when NK cells were incubated with iRBCs alone. (B) NK cells activation in the presence of iRBCs. Human NK cells were were purified (>95%) from fresh PBMC and co-cultured with synchronized schizont stage iRBCs at a parasitemia of 0.5% in 10:1 ratio (NK: iRBC) for 48h. Quantification of activation and degranulation markers was performed after 48 hours. NK cells co-cultured with iRBCs showed significant increase in the expression of CD25 and CD69, the two activation markers as well as for the expression of CD107a, a degranulation marker in comparison to the NK cells co-cultured with RBCs alone. Addition of anti-rabbit PfGBP-130 antibodies reduced the expression of both activation and degranulation markers in these NK cells in comparison to rabbit IgG isotype control. * Denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001.

    Article Snippet: The following antibodies were used for staining: anti-human CD3 (biotin, OKT3; Elabsciences), anti CD16 (3G8; Biolegend), anti CD11a (HI111; BioLegend), CD56-PE (5.1H11; Elabsciences), Ultra-LEAFTM Purified Human IgG1 Isotype (Biolegend), Goat anti-Human IgG Alexa FluorTM 488 (Invitrogen), Goat anti-Human IgG Secondary Antibody, Alexa FluorTM 594 (Invitrogen), (CD107a (H4A3; Elabsciences), CD25-Alexa Fluor® 488 (BioLegend), and CD69 (FN50; Elabsciences).

    Techniques: In Vitro, Cell Culture, Control, Incubation, Activation Assay, Purification, Expressing, Marker, Comparison